Title:

Misfolding, Unfolding and Folding

Speaker:

Dr. Matthew Lang

Affiliation:

Massachusetts Institute of Technology, Cambridge, MA

When:

Wednesday, March 31, 2010 at 3:00:00 PM   Add to Calendar

Where:

MRDC Building, Room 4211

Host:

Dr. Andres Garcia
andres.garcia@me.gatech.edu
404-894-9384

Abstract

Single molecule methods applied to molecular and cellular systems will be introduced. The first part of the talk will focus on misfolding in amyloid fibers including force fluorescence microscopy to measure their properties. Detailed studies of two molecular motors will follow: the ClpXP proteasome which recognizes proteins, mechanically unfolds, transports and degrades them and kinesin which transports cargo. The ClpXP protease, a member of the AAA+ class of motors, destroys protein substrates by unfolding them and coordinating machinery for recognition, denaturation, translocation and degradation, events which are measured a new single-molecule fluorescence assay using a series of fluorescently labeled GFP based substrates. Force generation in the kinesin motor protein is driven by folding between the N-terminal cover strand and neck linker to form the cover neck bundle, a mechanism that is directly tested by optical trapping measurements of engineered kinesin motor mutants.

Biography

No bio provided at this time.