SUMMARY

This work presents the application of microfluidics to manufacture lipid nanoparticles using regulated nanofluidic pulses for scalable manufacturing. Traditional methods of producing lipid vesicles lack the ability to achieve high-throughput and uniformity. Existing studies using pulsed jetting are capable of producing large lipid microparticles. However, the ability to achieve uniform nanoparticles using pulsed jetting has been very limited. A macro-to-microfluidics system was developed to create pulsed jet flows using micronozzles. Using the existing methods, a lipid bilayer is formed using a two-well chip and the micronozzle is placed in-plane with the bilayer. Upon pulsing the system, a jet flow from the micronozzle deforms the lipid bilayer creating a lipid vesicle. The ability to systematically combine multiple devices together allows for a scalable approach to manufacturing lipid particles.